Developmental stage of newly laid eggs (% total no. of eggs laid) | |||||||
---|---|---|---|---|---|---|---|
Genotype | One-to eight- cell | Nine-cell comma | Post- comma | No. of eggs laid | Locomation rate (body bends per min) | Unlaid eggs per adult | Pumping rate (pumps per min) |
N2 (wild type) | 6 | 94 | 0 | 189 | 21.5 ± 1.2 | 19.0 ± 1.2 | 230 ± 10 |
gpb-2(pk751) | 0 | 74 | 26 | 114 | 18.8 ± 1.5 | 32.1 ± 1.6 | 129 ± 8 |
egl-10(md176) | 0 | 0 | 100 | 134 | 2.9 ± 0.3 | 43.1 ± 1.1 | 238 ± 8 |
gpb-2(pk751);egl-10(md176) | 0 | 78 | 22 | 120 | 18.5 ± 1.7 | 31.3 ± 1.3 | 120 ± 7 |
eat-16(sy438) | 95 | 5 | 0 | 176 | 39.2 ± 1.1 | 3.8 ± 0.3 | 146 ± 6 |
gpb-2(pk751)eat-16(sy438) | 0 | 68 | 32 | 109 | 17.5 ± 1.6 | 31.0 ± 1.5 | 135 ± 6 |
eat-16(sy438);egl-10(md176) | 0 | 38 | 62 | 170 | 20.5 ± 1.0 | 30.0 ± 1.1 | 141 ± 7 |
At least 20 adult animals were assayed for each genotype (see materials and methods). Values reported are the means 6 standard errors. The eat-16(sy438) and egl-10(md176) alleles are putative loss-of-function mutations (Koelle and Horvitz 1996; HaiduCronin et al. 1999). ANOVA comparing groups for different variables was conducted. A significant effect of group was noted for locomotion (F = 75.9, P < 0.001), for unlaid eggs per adult (F = 142.5, P < 0.001), and for pharyngeal pumping (F = 44.5, P < 0.001). Differences between genotypes for the frequency of body bends during locomotion, unlaid eggs per adult, and pharyngeal pumping were analyzed using Student’s t-test. Differences from gpb-2(pk751) for the frequency of body bends during locomotion include: eat-16(sy438) (P < 0.001) and egl-10(md176) (P < 0.001). No significant difference was obtained for the frequency of body bends during locomotion between gpb-2(pk751) and N2 (wild type) (P < 0.064), gpb-2(pk751);egl-10(md176) (P = 0.44), gpb-2(pk751)eat-16(sy438) (P = 0.23), and eat-16(sy438);egl-10(md176) (P = 0.17). Differences from gpb-2(pk751) for the unlaid eggs per adult include: N2 (wild type) (P < 0.001), eat-16(sy438) (P < 0.001), and egl-10(md176) (P < 0.001). No significant difference was obtained for the amount of unlaid eggs per adult between gpb-2(pk751) and gpb-2(pk751);egl-10(md176) (P = 0.31), gpb-2(pk751)eat-16(sy438) (P = 0.30) and eat-16(sy438);egl-10(md176) (P = 0.11). The following differences were obtained for pharyngeal pumping: gpb-2(pk751) from N2 (wild type) (P < 0.001) and egl-10(md176) (P < 0.001). No significant differences were found for pharyngeal pumping between gpb-2(pk751) and gpb-2(pk751);egl-10(md176) (P = 0.18), gpb-2(pk751)eat-16(sy438) (P = 0.26), and eat-16(sy438);egl-10(md176) (P = 0.15).